Titlebook: Cell Culture Engineering IV; Improvements of Huma Barry C. Buckland,John G. Aunins,Weichang Zhou Book 1995 Springer Science+Business Media

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Intellectual

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homocysteine

Selecting and designing cell lines for improved physiological characteristics, natural variants with useful properties. Cholesterol independent variants of the mouse NSO myeloma cell line were isolated by cloning in a selective medium. A glutamine independent variant of a hyridoma was isolated by continuous (chemostat) culture under glutamine limited conditions in the presenc

口訣

Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal h experiments were performed in T-flasks using this model in supplemental medium design (Xie and Wang, 1994b). In this work, the major pathways of glucose and glutamine metabolism were incorporated into the stoichiometric model. Fed-batch culture was conducted in a 2-liter bioreactor with appropriat

ODIUM

Diverse effects of essential (,-6 and ,-3) fatty acids on cultured cells,ver, FAs have other effects on cells that may be less familiar. Polyunsaturated FAs of dietary origin (.-6 and .-3) cannot be synthesized by mammals, and are termed ‘essential’ because they are required for the optimal biologic function of specialized cells and tissues. However, they do not appear t

Glutinous

Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production,c acid on its own promoted cell growth when used at concentrations below 50 mg LP., but strongly inhibited growth at a concentration of 100 mg L. on more. However, linoleic acid bound to bovine serum albumin did not inhibit cell growth, even at a concentration as high as 100 mg L.. Also, linoleic ac

高談闊論

Growth rate suppression of cultured mammalian cells enhances protein productivity,ion, was examined for enhancing protein production by cultured mammalian cells. Hybridoma 2E3 cells which were adapted to be interleukin-6 sensitively growth-suppressed accumulated the mRNA of IgG. which is reported stable, and IgG. production rate increased as a result when their growth was suppres

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