Titlebook: Cartilage Tissue Engineering; Methods and Protocol Pauline M. Doran Book 2015 Springer Science+Business Media New York 2015 Bioreactors.Car

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Maxwell II. Modes and Mode Propagation, as well as the capacity for multilineage differentiation to bone, fat, and cartilage. Herein, we provide detailed methodologies for the derivation and characterization of potent mesenchymal-like progenitors from hES cells and describe in vitro assays for bone morphogenetic protein (BMP)-2-mediated differentiation to the chondrogenic lineage.

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Ontology Management Infrastructuresng scanning electron microscope and Picrosirius Red staining respectively. Methods to evaluate the metabolic activity and proliferation of cells (resazurin-based assay and DNA assay, respectively) and gene expression are also presented. Furthermore, histological techniques to analyze the presence of sulfated glycosaminoglycans are also described.

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Human Fetal and Adult ChondrocytesHuman adult, juvenile, neonatal, and fetal chondrocytes have all been demonstrated to produce cartilage matrix components in vitro for production of engineered tissues. In this chapter, procedures are outlined for isolation of chondrocytes from human fetal and adult cartilage. Methods for expansion

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Mesenchymal Stem Cells Derived from Human Bone Marrowability and multipotent character means that mesenchymal stem cells have become a focus for the field of tissue engineering, particularly for the repair of bone and cartilage..This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the ce

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Mesenchymal Stem Cells Derived from Human Adipose Tissuepacity of mesenchymal stem cells makes them very valuable for cell-based medical therapies. In this chapter, we describe how to isolate mesenchymal stem cells from human adult fat tissue, propagate the cells in culture, and cryopreserve the cells for tissue engineering applications. Flow cytometry m

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Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitorsdirected differentiation of human embryonic stem (hES) cells to the chondrogenic lineage. The initial enrichment of a hemangioblast intermediate has been shown to yield a replenishable population of highly purified progenitor cells that exhibit the typical mesenchymal stem cell (MSC) surface markers