Titlebook: Cancer Genomics and Proteomics; Methods and Protocol Narendra Wajapeyee Book 2014Latest edition Springer Science+Business Media New York 20

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A High-Throughput MicroRNA Expression Profiling System,m total RNA samples and on-site biochemical reactions, coupled with bead-based detection in 96-well format for hundreds of miRNAs per sample. With low-cost, high-throughput, high detection specificity, and flexibility to profile both small and large numbers of samples, this protocol can be adapted in a wide range of laboratory settings.

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CHAFE

Using LacO Arrays to Monitor DNA Double-Strand Break Dynamics in Live , Cells,cO arrays with targeting efficiencies as high as 70 %. Combining this facile LacO/LacI-GFP system with a site-specific, inducible DSB provides a means to monitor DSB dynamics at engineered sites within the genome.

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New Biophysical Methods to Study the Membrane Activity of Bcl-2 Proteins,lamellar vesicles and involves directly visualization of the process with a confocal microscope. This approach allows for the characterization of the membrane activity of the Bcl-2 proteins (or of any other pore-forming molecule) with unprecedented detail.

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apropos

Reduced Representation Bisulfite Sequencing to Identify Global Alteration of DNA Methylation,ucleotide level. DNA methylation status is determined by utilizing DNA methylation-specific restriction enzymes to selectively amplify for genomic regions that are rich in methylated DNA. Although the method is genome-wide, DNA methyl sequencing does not require the sequencing of the whole genome, h

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