Titlebook: Cancer Driver Genes; Methods and Protocol Timothy K. Starr Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 thr

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Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization. The protocols used to de/recellularize various organs and tissues are largely different. Here we describe a method to effectively engineer a bioartificial colon by completely removing original cells from human intestinal tissues followed by repopulating the acellular tissue matrix with cell cultur

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CRISPR/Cas9-Based Positive Screens for Cancer-Related Traitsnd the world have been working to make this data useful. Data like these can be made more useful by comparison with complementary . genomic data. One new example is the application of CRISPR/Cas9-based library screening for cancer-related traits in cell lines. Such screens can reveal genome-wide sup

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Ex Vivo Transposon-Mediated Genetic Screens for Cancer Gene Discoverygenetic screens have proven to be valuable for cancer gene identification, they are also time consuming and resource intensive. To facilitate the rapid and cost-effective identification of genes that regulate tumor-promoting pathways, we developed a complementary ex vivo transposon mutagenesis appro

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PiggyBac Transposon-Based Insertional Mutagenesis in Miceof epigenetically, transcriptionally or posttranslationally dysregulated genes remains a challenge. Transposon-based genetic screening in mice has proven to be a powerful approach to address this challenge. Insertional mutagenesis directly flags biologically relevant genes and, combined with the tra

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