Titlebook: Cancer Cell Signaling; Methods and Protocol David M. Terrian Book 2003 Humana Press 2003

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Functional Analysis of the Antimitogenic Activity of Tumor Suppressorssion. One is based on BrdU incorporation (i.e., DNA replication) and the other on flow cytometry. Together, this triad of techniques is sufficient to determine the action of tumor suppressors and other antiproliferative agents.

VAN

Signal Transduction Study Using Gene-Targeted Embryonic Stem Cellsetermine the role of the genes in the mediation of various cellular activities such as proliferation, differentiation, apoptosis, survival, transformation, and so on. Furthermore, with the recent advance of in vitro differentiation techniques, it is now feasible to rapidly determine the role of specific molecules in particular tissues.

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Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approache-modified antisense ODNs were transferred into prostate cancer cells with high efficiency, effectively inhibiting the expression of endogenous protein kinase C-epsilon and the androgen-independent (AI) proliferation of several independent human prostate cancer cell lines.

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Signal Transduction-Dependent Channelsnes by determining the retention of prostate-specific epithelial expression markers, including cytokeratins, the androgen receptor, estrogen receptor β and the dorsolateral prostatic secretory protein (mDLP) are given.

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NAG

Jonathan Buening,Rhonda Douglas Brownrotein tyrosine kinase/phosphatase molecules from cancer cells by reverse-transcription polymerase-chain-reaction. An improved profiling approach (RAGE) was also described by utilizing restriction enzyme digestion and electrophoresis for quick and efficient kinase/phosphatase profiling.

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Rescue and Isolation of ,-deficient Prostate Epithelium by Tissue Recombinationnes by determining the retention of prostate-specific epithelial expression markers, including cytokeratins, the androgen receptor, estrogen receptor β and the dorsolateral prostatic secretory protein (mDLP) are given.

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