Titlebook: Cancer Biomarkers; Methods and Protocol Gagan Deep Book 2022 Springer Science+Business Media, LLC, part of Springer Nature 2022 Precision m

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Scanning Electron Microscopy of Giant Cells from Giant Cell Tumor of Bone,d critical point dried (CPD) samples yield a greater number of isolated GCs with better surface morphology, including membrane folding and micro-vesicular structures on the surface. Collagenase digestion and CPD should be performed for ultrastructural surface imaging of individual giant cells.

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Immunofluorescence-Based Method to Assess Cancer Biomarker in the Hypoxic Region of the Tumor,ding necessary information about a cellular identity and the surrounding microenvironment. This chapter describes the utilization of immunofluorescence to identify such biomarkers in fixed tissue from prostate cancer (PCa) xenografts. One such marker detectable by immunofluorescence is pimonidazole,

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Zebrafish Xenograft Model to Study Human Cancer, and a wide range of pharmacologically active compounds elicit physiological responses in zebrafish embryos comparable to those in mammalian systems, making zebrafish ideal for identifying clinically relevant drug targets and compounds that regulate tumor progression. The zebrafish model is suitable

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In Vitro Cell Impedance Assay to Examine Antigen-Specific T-Cell-Mediated Melanoma Cell Killing to vival of cancer patients. Methods to examine the interaction of effector immune cells with target cancer cells are limited by labor-intensive labeling that can be examined at specific time points. In this report, we examine an antigen-dependent model of effector cytotoxic (CD8+) T-cell-mediated cyto