Titlebook: Calcium Signaling Protocols; David G. Lambert,Richard D. Rainbow Book 2013Latest edition Springer Science+Business Media, LLC 2013 Calcium

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Zeitschrift für Betriebswirtschaft of contractile function and cell signalling. Here the loading of cells either with an esterified fluorescence indicator prior to patch clamp recording, or dye loading via the patch pipette with “free” indicator, is described to allow simultaneous measurement of fluorescence and electrical signals.

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Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells of contractile function and cell signalling. Here the loading of cells either with an esterified fluorescence indicator prior to patch clamp recording, or dye loading via the patch pipette with “free” indicator, is described to allow simultaneous measurement of fluorescence and electrical signals.

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Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assayis is mixed with [.H]-labeled and unlabeled (generated from biological samples or standards) Ins(1,4,5)P.. Using the same principles as for radioimmunoassay/ELISA the mass of Ins(1,4,5)P. in biological samples can be estimated.

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