Titlebook: CRISPR-Cas Methods; M. Tofazzal Islam,Pankaj K. Bhowmik,Kutubuddin A. Book 2020 Springer Science+Business Media, LLC, part of Springer Na

小蟲

Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific Expressioependent of high transformation efficiencies. Here, we describe an updated protocol of ipGT for the model plant Arabidopsis, taking into account our recent improvements based on egg-cell-specific expression of . Cas9.

教唆

pulmonary

Homology-Directed Transgene-Free Gene Editing in ,possibility to purchase all necessary reagents commercially with no preparation time (besides design) facilitates rapid and routine genetic engineering in this organism. Here we describe the use of this technique to knockout locus ., which leads to rapamycin resistance and lends itself to an easy assay when adopting this gene-editing protocol.

Mingle

傳授知識(shí)

MORPH

Die Entfaltung der Wirbeltiere, system over other transformation methods. In this chapter, we describe a detailed protocol for generating . mutants via AMT to deliver single-guide RNA and Cas9, the components required for gene editing. This protocol can be used directly for CRISPR-Cas9-mediated gene editing of . and modified for other culturable oomycete species.