Titlebook: CRISPR Gene Editing; Methods and Protocol Yonglun Luo Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 CRISPR-C

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https://doi.org/10.1007/978-3-658-37228-6d clones. We also present our experiences of generating single nucleotide changes at 15 sites, which show considerable variability between both guides and target sites in the efficiency at which such changes can be introduced.

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1064-3745 idelines and methods for the vitally important CRISPR gene editing field.?.Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com..978-1-4939-9170-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER mutations by the genome editing method are largely dependent on the target site. As a consequence, the outcome of the editing operation is difficult to predict. Therefore, a quick assay to quantify the frequency of mutations is vital for a proper assessment of genome editing actions. We developed t

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Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAls, tissues, and whole animals showing great promise for translational applications. With regard to CRISPR/Cas9, a variety of repurposed systems have been developed to achieve gene knock-out, base editing, targeted knock-in, gene activation/repression, epigenetic modulation, and locus-specific label

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Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Virntal biological questions and to target and treat genetic diseases. The CRISPR system, originally derived from bacteria and archaea, can be delivered into cells using different techniques, comprising (1) transfection of mRNA or plasmid DNA, (2) electroporation of plasmid DNA or the Cas9 protein in a