Titlebook: Baculovirus; Methods and Protocol Manon M.J. Cox Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Baculovirus978-1-0716-3961-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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978-1-0716-3963-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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Construction of Recombinant Baculovirusesetic stability and high productivity, ideally within a short time period. Generation of recombinant baculoviruses requires the transfection of insect cells, harvesting of recombinant baculovirus pools, isolation of plaques, and the expansion of baculovirus stocks for their use for recombinant protei

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A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Bh in academia and industry. Since a baculovirus was first used to produce heterologous human IFN-β protein in insect cells, the BEVS has continuously been developed and its applications expanded. We have recently established a multigene expression toolbox (HR-bac) composed of a set of engineered bac

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Optimized Workflow from Gene to Productilizes a chemically defined, serum-free, protein-free, and animal origin free medium, making it suitable for recombinant protein expression experiments. The ExpiSf. chemically defined medium allows efficient transfection and baculovirus production directly within the same culture medium. The ExpiSf.