Titlebook: Bacterial Chromatin; Methods and Protocol Remus T. Dame Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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Genetic Engineering of , Using Competence-Induced Homologous Recombination Techniquesa . cell can initiate one out of various distinct differentiation processes to cope with changing environmental conditions. One of these differentiation processes is natural competence that allows cells to adsorb exogenous DNA and subsequently incorporate it into its chromosome by homologous recombi

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Atomic Force Microscopy Imaging and Analysis of Prokaryotic Genome Organizationimple cell manipulation procedure that enables stepwise dissection of the nucleoid. The procedure includes (i) on-substrate lysis of cells and (ii) enzyme treatment, followed by atomic force microscopy. This type of dissection analysis permits analysis of nucleoid structure ranging from the fundamen

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Atomic Force Microscopy Characterization of Reconstituted Protein-DNA Complexes straightforward method to characterize the binding behavior of different chromatin architectural proteins and to analyze the increasingly complex structural units assembled on the DNA. The protocol describes the preparation, AFM imaging, and structural analysis of chromatin that is reconstituted in

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Approaches for Determining DNA Persistence Length Using Atomic Force Microscopynical properties of DNA polymers depend on electrostatics and the strength of DNA base stacking by studying double-stranded DNA molecules incorporating several different neutral and charged base modifications. Here we describe ten complementary approaches for determining DNA persistence length by AF

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Book 2024Latest edition...?..Authoritative and cutting-edge,?.Bacterial Chromatin: Methods and Protocols, Second Edition .aims to be a?useful up-to-date reference work for researchers currently in the field and to those entering the field..

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Chavaunne T. Thorpe,Hazel R. C. Screenre (3C) methods revolutionized our ability to explore the hierarchical structure and the dynamics of bacterial genomes. Here, we review the major advances in the field of bacterial chromosome structure using 3C technology over the past decade.

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