Titlebook: Brown Adipose Tissue; Methods and Protocol David A. Guertin,Christian Wolfrum Book 2022 The Editor(s) (if applicable) and The Author(s), un

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Analysis of Enhancers and Transcriptional Networks in Thermogenic Adipocytes, brown adipocyte gene?programs can be predicted from chromatin accessibility and RNA-seq data. Furthermore, we demonstrate how information about target sites and target genes of the predicted key regulators can be integrated to propose testable hypotheses regarding the role and mechanisms of TFs.

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Signaling Lipidomic Analysis of Thermogenic Adipocytes, characterize the signaling lipidome can deconvolute the underlying biology of the lipid metabolites having a broad systemic impact on physiology. Herein, we describe how to perform and analyze LC-MS/MS signaling lipidomics on mature brown adipocytes.

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https://doi.org/10.1007/978-1-4039-1921-2em to activate Ca. cycling in an adipocyte-specific manner without external stimuli, i.e., fat-specific cold mimetics. Here, we describe the detailed methodology and application to the prevention of obesity.

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Book 2022ic adipocytes. The content reflects the advancing technologies in genetics, imaging, and ‘omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, which remain the core tenets of BAT biology. Written f

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https://doi.org/10.1007/978-1-349-89053-8 enzymatic detection of cellular fatty acid and glycerol efflux via fluorescent and colorimetric means, respectively. In addition, we also describe a genetically encoded luminescent detection system for intracellular fatty acids that is amenable to high-throughput analysis.

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Histories of Spousal Migration,chapter, we provide a detailed description of an optimized and feasible protocol to determine arteriovenous differences across iBAT. We include tips and practical advice for using this powerful tool to study BAT metabolism and secretory activity in rats as an experimental model.

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