Titlebook: Breast Cancer Research Protocols; Susan A. Brooks,Adrian Harris Book 2006 Humana Press 2006 Biopsie.Endoplasmatisches Reticulum.Migration.

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Immunohistochemistry over the years from a single layer of labeled antibody to amplified, labeled polymer-based systems, which enable low levels of antigen to be detected in different types of tissue preparation. However, alongside selecting the most appropriate methods of antigen recovery and enhanced signal amplifica

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Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistryestigate the presence of carbohydrate structures in or on cells, in much the same way as antibodies can be used to probe cells and tissues for the presence of specific antigens. As it is becoming increasingly apparent that subtle alterations in the glycosylation of cancer cells can profoundly influe

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Susan A. Brooks,Adrian HarrisIncludes supplementary material:

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https://doi.org/10.1007/978-3-642-99950-5e need for ultracentrifugation. This protocol produces total RNA that includes ribosomal, transfer, and messenger RNA..This high-quality RNA is suitable for Northern blot analysis, dot-blot hybridization, poly (A) RNA selection, in vitro translation, cDNA library construction, reverse transcriptase-

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H. Huber,G. Michlmayr,Ch. Hubererful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent sodium dodecyl sulfate. Ethylenediaminetetraacetic acid is included in the lysis buffer to inhibit DNases.

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