Titlebook: Brassinosteroids; Methods and Protocol Eugenia Russinova,Ana I. Ca?o-Delgado Book 2017 Springer Science+Business Media LLC 2017 Plant hormo

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Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues,ocesses, including root and shoot growth, vascular differentiation, fertility, and seed germination. A characteristic feature of all plant hormones, including BRs, is that their concentration is extremely low in plant tissues and, therefore, the methods dealing with their determination belong to ult

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Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability be a method to observe changes in protein stability under different light conditions. In brief, . seedlings were maintained under various light regimes from continuous light to total darkness or transitions from light to dark, whereafter total protein was extracted from plants. Proteins were measure

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Approaches to Study Light Effects on Brassinosteroid Sensitivity, responses by altering hormone concentration, tissue sensitivity, or a combination of both. Whereas it is relatively straightforward to assess the light effects on hormone levels, hormone sensitivity is subjected to interpretation. In . seedlings, hypocotyl length is strongly affected by light condi

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Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-ThSUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-through

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Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Assone expression, has therefore been the focus of many studies. These analyses have been primarily conducted on total extracts derived from a mixture of tissues and cell types, consequentially limiting delineation of precise models. In this chapter, methods for tissue-specific functional genomics are o

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