Titlebook: Brain Tumor Stem Cells; Methods and Protocol Sheila K. Singh,Chitra Venugopal Book 2019 Springer Science+Business Media, LLC, part of Sprin

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Apoorva S. Shastri,Kailash Shaw,Mangal Singhrapy, and relapse. Understanding how these populations of cells expand in response to a host of conditions is critical in determining effective cancer therapeutics. A defining feature of cancer stem cells is the ability to switch between modes of quiescence and symmetric/asymmetric division to prote

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https://doi.org/10.1007/978-3-030-93736-2o proteins of interest, including transcription factors, co-factors, or chromatin remodeling proteins. These isolated DNA fragments may include gene regulatory regions from enhancers, super-enhancers, promoters, and/or insulators. Cells of interest can be obtained from embryonic tissues at various d

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Timon Sachweh,Daniel Boiar,Thomas Liebiggnificant therapeutic benefits for therapeutic evaluation. For the identification of such an elusive and rare subpopulation of cells, a single cell analysis technology with deep profiling capabilities known as Mass Cytometry (CyTOF) can prove to be highly useful. CyTOF circumvents the spectral overl

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https://doi.org/10.1007/978-3-030-93736-2s9 system has now successfully been applied for genetic screens in many cell types, providing a powerful tool for functional genomics with manifold applications. Genome-wide guide-RNA (gRNA) libraries allow facile generation of a pool of cells, each harboring a gene knockout mutation that can be use

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Timon Sachweh,Daniel Boiar,Thomas Liebigth the development of the cancer stem cell (CSC) hypothesis, it has been postulated that a rare, slow dividing tumor cell population is able to escape therapy and contribute to tumor relapse and metastasis. The advances in characterization of CSCs across multiple cancer subtypes have allowed for dev

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https://doi.org/10.1007/978-3-031-23633-4There is a desperate need for a better understanding of the basic biology of these tumors, in order to devise novel, more specific and effective therapeutics. The handling of GBM represents a daunting challenge to clinicians, also considering the few therapeutic options available, none of which can

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Niken Prasasti Martono,Hayato Ohwadaortant to express the mutated genes at appropriate levels, in relevant cell types, and in the proper developmental context. For recurrent mutations found in the heterozygous state in tumors, expression of the mutation from the endogenous locus is a more physiologically relevant recapitulation of the

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What to?Do with?Your Sentiments in?Financeechanisms of metastatic development as well as a clinically relevant therapeutic screening platform. Here we describe the development of a novel mouse model of brain metastasis from a primary lung cancer utilizing primary patient samples. These models provide an accurate representation of different

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Introduction to Brain Tumor Stem Cells,res the history of normal and cancerous stem cells, and their implication in the current model of brain tumor development. The origins of stem cells date back to the 1960s, when they were first described as cells capable of self-renewal, extensive proliferation, and differentiation. Since then, many