Titlebook: Brain Development; Methods and Protocol Simon G. Sprecher Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer Na

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A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Em genesis is driven by specific gene products whose expression underlies a tight spatiotemporal control. Therefore, the analysis of gene expression in time and space provides valuable insights into the molecular mechanisms that govern brain development. Since .-specific antibodies are scarce, fluores

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Immunostainings in Nervous System Development of the Nematode nslucence, short life cycle, and abundance of genetic tools (WormBase. ., 2018; WormBook. The C. elegans Research Community. ., 2018). Due to the relative ease of genetic transformation, the majority of studies in . use transgenes (e.g., green fluorescent proteins) to assess the expression and distr

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