Titlebook: Brain Development; Methods and Protocol Simon G. Sprecher Book 2020Latest edition Springer Science+Business Media, LLC, part of Springer Na

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Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian c reporter lines in which fluorescent proteins are expressed in defined populations of neurons are important tools that can overcome these difficulties. By using membrane-attached fluorescent proteins, such reporter transgenes can identify the complete outline of subsets of neurons or they can highl

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Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System. Understanding how organ systems and tissues develop and the molecular underpinnings of the processes involved has become an area of new research. The microscopic anatomy of these organisms is best understood through the systematic use of immunochemistry and in situ hybridization procedures. These

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Flybow to Dissect Circuit Assembly in the , Brain: An Updatesms that control neural circuit development and function. This chapter provides detailed technical information on how to use . variants of the mouse Brainbow-2 system, called Flybow, for stochastic labeling of individual cells or lineages with different fluorescent proteins in one sample. We describ

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Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brainto keep the cells under best physiological condition and to prevent abnormal cellular behavior, which might be caused by phototoxicity during microscopy. In this chapter we describe a protocol to visualize division patterns of neural stem cells in live whole mount brains of . larvae. We also present

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CRISPR/Cas9 Genome Editing to Study Nervous System Development in nervous system. While transgenic approaches have been heavily used to investigate how the brain develops, genome editing has been notoriously hard in the fruit fly. The advent of versatile CRISPR/Cas9-based genome editing techniques allow the generation of engineered loci using homologous repair to