Titlebook: Bleomycin: Chemical, Biochemical, and Biological Aspects; Proceedings of a joi Sidney M. Hect Conference proceedings 1979 Springer-Verlag N

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Biogenetic Aspects of Bleomycin-Phleomycin Group Antibioticsidine, and (v) the mechanism of glycoside formation. The first problem has already been solved and reported (9). As an approach to solution of the other problems, we chose to isolate intermediate peptide fragments from the culture medium. The exciting results obtained, which exceeded our expectation

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Liquid Chromatography of Bleomycinlar products. Moreover, the retention time of a given compound on LC is sometimes useful for predicting what modification has occurred, (iv) The purity of BLM and its modification products can be verified by high-performance liquid chromatography (HPLC).

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The Metallobleomycinsn and in so doing apparently inactivate the drug. The addition of Pe(II)salts to the reaction mixture, on the other hand, under conditions which lead to.the formation of the Fe(II)-BLM complex, greatly enhances the DNA degradingability of bleomycin..

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The Use of Covalently Closed Circular DNA to Investigate Properties of Bleomycin and Its Analogs fluorescenceresults upon alkaline denaturation of broken eee DNA. This provides the basis for a fluorescent assay of DNAbreakage (Fig. I). Bleomycin-induced DNAfragmentation can also be detected using electrophoresis of eeeDNA. This is because the products of broken superhelicaleee DNA(form I), rel

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