Titlebook: Biotechnologies for Plant Mutation Breeding; Protocols Joanna Jankowicz-Cieslak,Thomas H. Tai,Bradley J. Book‘‘‘‘‘‘‘‘ 2017 International A

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Free-Radical

Chemical and Physical Mutagenesis in ,ack of genetic variability hampers the breeding progress, mutagenesis can introduce genetic variation, reveal gene function, and aid in the characterization of candidate genes involved in biological functions. Mutagenized populations are useful for screening for altered phenotypes and physiological

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Creation of a TILLING Population in Barley After Chemical Mutagenesis with Sodium Azide and MNUies. The creation of an appropriate population is the first and most crucial step of TILLING. The goal is to obtain a highly mutagenized population that allows many mutations in any gene of interest to be found. Therefore, an effective method of mutation induction should be developed. A high mutatio

難管

Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollens (TALENs) is provided. As explant for the introduction of TALEN expression constructs, embryogenic pollen cultures are used which consist primarily of haploid cells able to be converted via pollen embryogenesis and genome duplication into fertile doubled haploid plants. Thanks to the haploid nature

Hirsutism

Doubled Haploidy as a Tool for Chimaera Dissolution of TALEN-Induced Mutations in Barleyties of crop improvement. In the approach described in this chapter, the two units of transcription activator-like effector nucleases (TALENs) required for site-directed cleavage activity are separately used to produce homozygous transgenic lines, each carrying only one unit of the TALEN pair. Cross

閑蕩

Field Evaluation of Mutagenized Rice Materialp new varieties of crops and ornamentals. More than 3200 varieties have been produced through mutation induction, and these have contributed towards improving food security in many countries..Proper field techniques can greatly enhance the chances of identifying desirable phenotypes. The protocol de

乞丐

Root Phenotyping Pipeline for Cereal Plantsereal plants. The designed pipeline is composed of a drip irrigation system to supplement plants with a medium, a high-resolution root system scanning facility and a method for comprehensive image analysis. The system enables low-effort, accurate and highly repeatable analysis of features of the roo