Titlebook: Biophysical and Biochemical Aspects of Fluorescence Spectroscopy; T. Gregory Dewey Book 1991 Springer Science+Business Media New York 1991

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Grasping

Continuous, On-Line, Real Time DNA Sequencing Using Multifluorescently Tagged Primers,DNA sequencing is a relatively new technique, just over a decade old. Scientifically, it is a very important technique because the DNA sequence gives us the ultimate resolution of the genetic material to the single base level and thus its complete information.

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Time-Resolved Fluorescence in Biology and Biochemistry,ctroscopy in the chemical and biological sciences (Badea and Brand, 1979; Demas, 1983; Lakowicz, 1983; Cundall and Dale, 1983; Gratton ., 1984a; Jameson ., 1984; Taylor ., 1987; Jameson and Reinhart, 1989). Our goal in this chapter is thus not to treat the subject exhaustively but rather to reiterat

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Analysis of Ligand Binding and Cross-Linking of Receptors in Solution and on Cell Surfaces,of this method is that nanomolar concentrations of fluorophores can be detected in the presence of a high level of background noise provided by the cells. An ideal fluorescent probe absorbs and emits at wavelengths not in common with the cellular components, has a high quantum yield, and can be plac

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Imaging Membrane Organization and Dynamics,ry after photobleaching (FRAP) can be extended, by coupling these techniques to low-light-level video microscopy and digital image processing, to provide an additional dimension of information. That is, what new information can be gained when, rather than integrating the fluorescence signal over a p