Titlebook: Biophysical Osteoblast Stimulation for Bone Grafting and Regeneration; From Basic Science t Nahum Rosenberg Book 2023 The Author(s), under

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The Clinical Potential of the In Vitro Generated Bone-Like TissueBone has a high potential to regenerate after damage; however, the efficacious repair of large defects resulting from resection, trauma, extended fractures, or inadequately vascularized bone gaps will not achieve satisfactory healing and will be considered non-union [1] (Fig. 5.1).

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978-3-031-06922-2The Author(s), under exclusive license to Springer Nature Switzerland AG 2023

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https://doi.org/10.1007/978-3-322-85846-7ical (mechanical) stimulation of the osteoblasts. Furthermore, this tissue has no toxic or tumorigenic potential. The primary use of such tissue is to bridge critical gaps in bone, i.e., to be used as efficient bone graft material.

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Ramanspektroskopie in Halbleiternlast proliferation and maturation. Therefore, EMF is used to treat delayed union or non-union in bone fractures. This method evolved from in vitro (see Part I) and animal studies to an established clinical modality, which is currently approved by the regulatory authorities [1].

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Anwendungen der Laserspektroskopie,cal simulation methods. Mechanical vibration, alternating and pulsed electromagnetic fields, and pulsed photobiomodulation can be used for such purposes. Several versatile standard experimental concepts have been described previously and are used primarily in this type of research.

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Anwendungen der Laserspektroskopie, in monolayer culture to induce proliferation, maturation, and matrix elaboration by applying alternating external biophysical stimulation with specific optimal parameters (frequency, amplitude) for each cellular activity. For this purpose, the bioreactor should contain cells seeded on an osteocondu

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