Titlebook: Biomembrane Protocols; II. Architecture and John M. Graham,Joan A. Higgins Book 1994 Springer Science+Business Media New York 1994

青春期

covert

acrimony

烤架

Olaf Kühne,Karsten Berr,Petra Lohmannwealth of experience accumulated in the course of the crystallization and structural analyses of several hundred soluble globular proteins and their complexes, the process of crystallization still remains something of an art, and is often the rate limiting step of any analysis. For membrane proteins

Herd-Immunity

Olaf Kühne,Karsten Berr,Petra Lohmannpartmentalization of function is established by the maintenance of asymmetric structure and function between the apical and basolateral surfaces of the cell. This asymmetry (polarity) extends on a biochemical level to the membrane proteins of the apical and basolateral plasma membrane. Indeed, many

NUL

神圣將軍

Hyaluronic-Acid

Karl-Otto Wenkel,Alfred Schultz,Gerd Lutzes with a myeloma cell line; selection, cloning, and freezing of hybridoma cell lines; and screening for monoclonal antibody production. Part A of this chapter will be concerned with the production of hybridoma cell lines, and Part B with the screening procedures.

craven

Gerd Lutze,Alfred Schultz,Karin Luzition of membrane proteins has been achieved without the use of detergents (e.g., by sonication of membrane proteins with lipids), detergent-based methods are more widely used and appear to be more reproducible (.). If a particular protein is to be purified in an active form, then particular attentio

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