Titlebook: Biomarker Methods in Drug Discovery and Development; Feng Wang Book 2008 Humana Press 2008 Clinical sample handling.DNA.Drug efficacy and

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Single Nucleotide Polymorphisms and DNA Methylation Analysis Using Pyrosequencing Methods,l controls for each sample. Pyrosequencing can also be used to identify triallelic, indel, and short-repeat polymorphisms, as well as determining allele percentages for DNA methylation or pooled sample assessment. Assays details for pyrosequencing of clinically relevant polymorphisms and DNA methylations are described in this chapter.

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https://doi.org/10.1007/978-3-662-08149-5icular TaqMan.-based real-time PCR gene expression assays, provides a simple, robust, and practical tool that has shown great potential in bridging the gaps between biomarker discovery and clinical practice. In this chapter, we will survey the principle of this technology and its wide applications in biomarker discovery and validation.

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Rahmenbedingungen der Europ?isierung, ProteinChip arrays can be designed to characterize antibody-antigen and other protein-protein interactions. This review will update the reader on the current SELDI methods and protocols that are especially pertinent to protein biomarker discovery.

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https://doi.org/10.1007/978-3-531-90009-4ivariate statistical methods are used to explore the complex metabolite patterns of biofluids. In this chapter, the methodology and application of high-performance liquid chromatography (HPLC)-MS–based metabonomics in biomarker discovery are reviewed.

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https://doi.org/10.1007/b138580capable of handling a large amount of data are essential in order to reduce the cost of discovery and increase the throughput. In this chapter, we will discuss and describe a case study of a unique proteomic method that uses a non-gel, label-free LC/MS-based protein quantification technology.

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