Titlebook: Bioluminescent Imaging; Methods and Protocol Christian E. Badr Book 2014 Springer Science+Business Media New York 2014 Aequorin.Bioluminesc

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Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell CultureMycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the . luciferase reporter.

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Julia Storch,Mike H?nsel,Matthias Hüblercell viability, proliferation, death, and energy transmission. In this chapter, we describe an improved and efficient method for extraction of ATP from tissues and cells using phenol-based reagents. The chaotropic extraction reagents reported so far co-precipitate ATP with insoluble proteins during

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Transfusion von Blut und Blutderivatenmay continue to signal from endosomes. Accessibility of cell surface receptors and the central function of ligand–receptor binding in signal transduction make ligand binding a prime target for therapeutic agents. We describe a . luciferase complementation method for imaging ligand–receptor binding i

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Allgemeine Ursachen für Komplikationense small RNAs have been demonstrated to play crucial roles in a variety of biological processes including cell differentiation, proliferation, and survival. Knowledge of specific expression patterns of miRNAs is critical for functional studies. Here, we describe a bioluminescence reporter gene-based

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,Fall 5?– Die postoperative Verlegung,bricate bioluminescent capsules for illuminating intracellular signaling and cargo protein delivery. The capsule consists of four components, which are, in consecutive order: a secretion peptide (SP), a host luciferase body (leader), a guest protein or peptide (cargo), and a membrane localization si

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Peer W. K?mmerer,Bilal Al-Nawasimeric protein consisting of HIF-1α oxygen-dependent degradation domain (ODD) fused to luciferase was ubiquitously expressed in all tissues. Hypoxic stress leads to the accumulation of ODD-luciferase in the tissues of this mouse model which can be identified by noninvasive bioluminescence measuremen