Titlebook: Bioluminescence; Methods and Protocol Sung Bae Kim Book 2016Latest edition Springer Science+Business Media New York 2016 luciferase.optical

homeostasis

Monitoring Intracellular pH Change with a Genetically Encoded and Ratiometric Luminescence Sensor inin exhibits pH dependence in vitro. When expressed in either yeast or mammalian cells, pHlash reports basal pH and cytosolic acidification. In this chapter, we describe an in vitro characterization of pHlash, and also in vivo assays including in yeast cells and in HeLa cells using pHlash as a cytoplasmic pH indicator.

出來

褻瀆

Live Cell Bioluminescence Imaging in Temporal Reaction of G Protein-Coupled Receptor for High-Througcently developed a bioluminescence method for the quantitative detection of the interaction between GPCR and β-arrestin using split luciferase complementation. To monitor time-course variation of the interactions, a new imaging system contributes to the accurate evaluation of drugs for GPCRs in a high-throughput manner.

Legion

Foreknowledge

N防腐劑

Zusammenfassung und Schlussfolgerungenn optical readout, including live cell imaging, single-chain probes, and bioluminescent tags of antibodies. The present protocol guides on how to fabricate a unique artificial luciferase with designed optical properties and functionalities.

嘮叨

exceptional

Molecular Cloning of Secreted Luciferases from Marine Planktonic Copepodstified copepod luciferases from bioluminescent copepod crustaceans. Here, we introduce how to establish a cDNA encoding novel copepod luciferases from a copepod specimen by PCR with degenerated primers.