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Titlebook: Bioluminescence; Methods and Protocol Sung-Bae Kim Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive

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發(fā)表于 2025-3-23 11:55:23 | 只看該作者
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Method for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Ithe UCBR concentration is easily determined through measuring the variance in the BL color with a smartphone camera. This method provides a practical method to lead to future point-of-care diagnosis with quick and simple procedures.
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Single-Cell NanoBRET Imaging with Green-Range HaloTag Acceptorapplications, mainly due to the low signal output resulting in poor signal-to-noise ratio. In this chapter, we describe a protocol to optimize spatiotemporal BRET imaging by adopting fluorescent HaloTag acceptors, adapting cell culture conditions and microscopic setup.
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發(fā)表于 2025-3-24 15:01:29 | 只看該作者
Book 2022Latest editionund the world. Volume 2 delves into techniques for heterogeneous conjugates, protein fragment-complementation assays, BRET-based imaging, as well as instrumentation and software. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respecti
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https://doi.org/10.1007/978-3-531-91181-6the host cells. Although BLI has such superior properties over FLI, the available wavelengths in BLI are mostly limited to the visible region. Here we present bioluminescence resonance energy transfer (BRET)-based visible and near-infrared dual-color molecular imaging using a quantum dot (QD) and luciferase–protein conjugate.
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