Titlebook: Biolistic DNA Delivery in Plants; Methods and Protocol Sachin Rustgi,Hong Luo Book 2020 Springer Science+Business Media, LLC, part of Sprin

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https://doi.org/10.1007/978-3-531-90483-2y, versatility, and high efficiency. It has been a preferred method for investigating plant gene function in most monocot crops. The first transgenic sorghum plants were successfully regenerated through biolistic DNA delivery in 1993, with a relatively low transformation efficiency of 0.3%. Since th

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Toxoid-Vaccines

https://doi.org/10.1007/978-3-663-08069-5ted 12–16?days post-anthesis, the embryonic axis is removed, and the immature scutella are precultured for 1–2?days prior to particle bombardment. Gold particles are coated with plasmid DNA containing the gene(s) of interest plus a selectable marker gene, in this instance . (bialaphos resistance), a

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任命

https://doi.org/10.1007/978-3-531-90904-2ists, especially in the era of genome editing. The ability to deliver proteins or protein/RNA complexes into cells allows for producing gene-edited plants that are free of transgene integration in the genome. Here we describe a protocol for the delivery of a protein/gold particle mixture in plant ce

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