Titlebook: Bio-Carrier Vectors; Methods and Protocol Kumaran Narayanan Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Ba

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Scaffold-Based Delivery of CRISPR/Cas9 Ribonucleoproteins for Genome Editingmobilize biomolecules on the surface. Nanofibers will provide a biomimicking microenvironment and topographical cues to seeded cells. For evaluation, a model cell line with single copies of enhanced green fluorescent protein (U2OS.EGFP) will be used to validate the efficiency of gene disruption.

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Kumaran NarayananIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/186326.jpg

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Edition and Translation Principlesuman cells presenting β1 integrin receptors. This technique is relatively underutilized owing to the inefficiency of gene transfer to targeted cell populations. Bacterial vectors must successfully adhere to the cell membrane, internalize into the cytoplasm, undergo lysis, and deliver DNA to the nucl

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https://doi.org/10.1007/978-3-7643-8391-6escence microscopy. These microscopes can provide basic information on the invasiveness of the bacterial vector including the ability of the vector to successfully adhere to the cell membrane. Standard microscopy techniques however fall short when finer details including membrane attachment as well