Titlebook: Bifidobacteria; Methods and Protocol Douwe van Sinderen,Marco Ventura Book 2021 Springer Science+Business Media, LLC, part of Springer Natu

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Identification of Bifidobacteria by the Phosphoketolase Assay,g to their health and well-being. An important taxonomic marker for the identification of members of the bifidobacterial group is the presence of the fructose-6-phosphate phosphoketolase (F6PPK) activity. The F6PPK enzyme is involved in the bifidus shunt based on the ability of F6PPK to split fructo

Euthyroid

Determination of Bile Salt Hydrolase Activity in Bifidobacteria,salt hydrolysis is considered to represent a bile detoxification mechanism for gut commensal bacteria and thus the presence of this activity was believed to be a predictor of bile tolerance of putative probiotic strains. On the other hand, it has recently been revealed that chemical modifications of

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Metagenomic Analyses of Bifidobacterial Communities,s have been shown to be correlated with many aspects of host health by means of direct interactions with the host and cohabiting microbes. Metagenomic sequencing of fecal DNA represents a valuable approach for taxonomic and functional profiling of bacterial populations, and has allowed us to appreci

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Resistance of Bifidobacteria Toward Antibiotics,t safety record. However, in the context of the increasing worldwide problems associate to the rise of pathogenic microorganisms with acquired resistance to antibiotics, the risk associated to the presence of antibiotic resistance determinants should always be a key starting point for the introducti

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Methods for Isolation and Recovery of Bifidobacteria, be encountered within the sample and the particular metabolic traits of the bifidobacterial species adapted to such inhabitation..A wide array of culture media recipes have been described in the literature to routinely isolate and grow bifidobacteria under laboratory conditions. However, there is n

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Site-Directed Mutagenesis of , Strains, achieved using a nonreplicating vector, harboring a DNA fragment corresponding to an internal part of the gene to be mutated. This vector is introduced into a bifidobacterial cell of the strain in question by electroporation. Through homologous recombination, this vector is integrated into the geno