Titlebook: Behavioral Neurogenetics; Daisuke Yamamoto Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer

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In Vivo Brain Imaging in Freely Moving and Socially Interacting ,,isplay highly complex behaviors. One outstanding challenge in . neuroscience is to monitor neural activity in untethered, freely behaving animals. Here, we describe a method named Flyception2, which allows for real-time brain activity?imaging from a freely walking and socially interacting fly. We pr

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Automated Behavior Analysis Using a YOLO-Based Object Detection System,mposed of two steps of data processing: tracking and behavior annotation. The former generates the time series of positional data describing the movement of individuals and/or body parts, which is subsequently analyzed in the latter to identify particular behaviors/actions using the mathematically d

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Proteomic Analysis of , Neurons Using TurboID-Based Proximity Labeling,chines. Here, we review a proximity-labeling strategy that uses the improved . biotin ligase TurboID to characterize . protein complexes. Although the focus is on . neurons, the method is applicable regardless of cell type. We describe detailed extraction procedures that solubilize the bulk of . pro

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0893-2336 ation advice from the expertsThis volume looks at the framework in which different scientific disciplines are integrated into the latest studies in behavioral neurogenetics, and covers new approaches toward understanding the system that controls behavior across the animal kingdom. Chapters in this b

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Combined in Situ Hybridization Chain Reaction and Immunostaining to Visualize Gene Expression in Wh. species and in the validation of single-cell RNA-Seq results in the fly nervous system. Our protocol provides a simple, robust, multiplexable, and relatively affordable means to quantitatively visualize gene expression in the nervous system of flies, facilitating its general use in fly neurogenetic studies.

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Mapping Brain-Wide Mesoscale Connectome from Single Animals with BRICseq, for high-throughput mapping of brain-wide mesoscale connectome from single animals. In combination with other methods to map brain-wide gene expression or neural activity, BRICseq allows comparative and integrative studies of the nervous system across animal models of various manipulations, diseases, and species.

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