Titlebook: Basic and Advanced Laboratory Techniques in Histopathology and Cytology; Pranab Dey Textbook 2022Latest edition The Editor(s) (if applicab

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Cytology Sample Procurement, Fixation and Processingd commonly by direct smear, centrifugation, cytocentrifugation, liquid-based preparation, millipore technique and cell block. This chapter presents a detailed description of these various techniques of processing the sample.

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self-assessment.Provides comprehensive and easy to follow d.The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special la

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High Performance Memory Testinganionic dye. When a metallic salt (mordant) is combined with haematin, then a cationic dye-metal complex is formed that behaves as a strong basic dye and combines with the nucleic acid. This chapter discusses the different types of hematoxylin solutions, their uses, and the staining procedure.

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Haematoxylin and Eosin Stain of the Tissue Sectionanionic dye. When a metallic salt (mordant) is combined with haematin, then a cationic dye-metal complex is formed that behaves as a strong basic dye and combines with the nucleic acid. This chapter discusses the different types of hematoxylin solutions, their uses, and the staining procedure.

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Stains for the Microbial Organismss are required to demonstrate the morphology and characteristic colour of the organisms. This chapter describes the essential stains to demonstrate the various microorganisms in the histopathology and cytology material. The principle of stains, the basic ingredients of the staining solution and the steps of staining have been described here.

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Fixation of Histology Samples: Principles, Methods and Types of Fixativesn, advantages, and disadvantages of the fixatives are discussed. The various factors affect the fixation rate such as pH, temperature, concentration, agitation etc. The chapter discusses the different types of fixatives used for different tissues for different purposes. The various fixation artefacts are also discussed.

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Routine Staining in Cytology Laboratoryery good cellular differentiation and nuclear details. This chapter presents the principle and steps of the routine stains in the cytology laboratory. It also describes the various precaution to avoid the problems of staining and particular emphasis have been given to Pap stain.

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