Titlebook: Basic Techniques in Molecular Biology; Stefan Surzycki Book 2000 Springer-Verlag Berlin Heidelberg 2000 DNA.DNA Isolierung.DNA sequencing.

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Challenges of Future High-End Computingrch. The Southern hybridization procedure described in this chapter is separated into two general protocols: transfer of the DNA to the membrane and hybridization of immobilized DNA to DNA or RNA probes. A description of agarose gel electrophoresis of DNA is given in Chapter 8.

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https://doi.org/10.1007/978-1-4615-5611-4he vector into bacterial cells and amplifying vector DNA using bacterial DNA replication machinery. The DNA fragment, or insert, can be derived from any organism and obtained from genomic DNA, cDNA, previously cloned DNA, PCR or synthesized in vitro.

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PCR Analysis,st. The technique has revolutionized molecular biology and is used in virtually every area of natural sciences and medicine. The technique has cut across the boundaries separating basic and applied research, commercial technology and medicine in a way few techniques ever have.

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Book 2000hanisms of each step. This knowledge will enable the users to design their own modifications or to adapt the method to different systems..Surzycki has been teaching undergraduate courses and leading workshops for many years, during which time he has extensively modified and refined the techniques he has described in this manual.

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DNA Transfer and Hybridization,rch. The Southern hybridization procedure described in this chapter is separated into two general protocols: transfer of the DNA to the membrane and hybridization of immobilized DNA to DNA or RNA probes. A description of agarose gel electrophoresis of DNA is given in Chapter 8.