Titlebook: Basic DNA and RNA Protocols; Adrian J. Harwood Book 1996 Humana Press 1996

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Hybridization in Flow Cytometry) have been described in the preceding chapters (.. and .). This chapter describes the detection of specific DNA sequences by hybridization to a labeled probe of complementary sequence. This method is suitable for the detection of a wide range of DNA concentrations down to single-copy genes within m

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Methods in Leukocyte Cytochemistryhese methods offer an attractive alternative to “radioactively tagged” probes in terms of safety, cost, and efficiency. Most previous nonradioactive strategies utilized the detection of the modified base by the use of a coupled antibody- or avidin-alkaline phosphatase with subsequent exposure to Nit

Minatory

Quantitative Fluorescence Cytometryn. Such molecules are generally coupled to a nucleoside triphosphate, such as dUTP, via a linker group. The modified nucleotide is then incorporated into the probe by an enzyme-mediated reaction, such as random prime labeling for long probes or terminal transferase-catalyzed 3′-end labeling for olig

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Methods in Leukocyte Cytochemistry to the researcher. The labels generally fall into one of two categories: primary labels, in which a detectable signal, such as a radioisotope or a fluorophore, is introduced directly into the probe, and enzymatic labels, in which an enzyme is used to catalyze a signal-generating reaction. In the la