Titlebook: Basic Concepts on 3D Cell Culture; Cornelia Kasper,Dominik Egger,Antonina Lavrentieva Textbook 2021 The Editor(s) (if applicable) and The

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https://doi.org/10.1007/978-3-030-57299-0 been developed decades ago and are still being used routinely. However, they may to some extent be harmful to the cultured cells and alter phenotype, functional characteristics, and potency as compared to the same cell type residing in the body. This is of crucial relevance during translational dev

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Disambiguating Kant for the Sake of Science,ene and ultimately protein expression in comparison to conventional 2D cultivation. There is a wide variety of materials available for the 3D cultivation of cells, ranging from hydrogels that can be used to encapsulate cells, to porous 3D matrices, which can be seeded with cells. In this chapter, we

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L. J. S. Harrison,G. W. P. Joshuaysiologically relevant cell culture conditions, the surrounding environment must be controlled by emerging microfluidic systems. Thus, in the first part of this chapter we will learn about the tremendous benefits of microfluidic devices, their fabrication, and finally their implementation in novel a

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https://doi.org/10.1007/978-94-009-9972-5 Raman trapping microscopy coupled with multivariate statistical analysis, to monitor individual cells in 2D cultures and 3D tissues. We shortly describe the concept of spontaneous Raman spectroscopy using a bio-compatible 785 nm laser and the advantages of combined Optical Trapping. Furthermore, yo

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