Titlebook: Base Excision Repair Pathway; Methods and Protocol Kishor K. Bhakat,Tapas K. Hazra Book 2023 The Editor(s) (if applicable) and The Author(s

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Highly Sensitive Radioactivity-Based DNA 3′-Phosphatase Activity Assay for Polynucleotide Kinase 3′-air process. Here we describe two novel assay systems to detect phosphate release by PNKP’s 3′-phosphatase activity and PNKP-mediated in vitro single-strand break repair with minimal repair components (PNKP, DNA polymerase, and DNA ligase) using either purified proteins or cell-free nuclear extracts

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In Vitro Reconstitutive Base Excision Repair (BER) Assayed in the base excision repair pathway. In the present chapter, we describe the detailed methodology to reconstitute base excision repair assay systems. These reconstitutive BER assay systems use artificially synthesized and modified DNA. These reconstitutive assay system will be a true representati

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Slot Blot Assay for Detection of R Loops?not always?useful for initial assessments of R loop accumulation. Here, we describe an improved slot blot protocol to detect and estimate R loops and show its sensitivity and specificity using the S9.6 antibody. Since specific factors protecting cells from harmful R loop accumulation are expanding,

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Characterizing the Repair of DNA Double-Strand Breaks: A Review of Surrogate Plasmid-Based Reporter or oligonucleotide-based substrates contain restriction enzyme-cleaved DSB mimics, which undoubtedly do not mimic DSB ends generated by ionizing radiation (IR), chemotherapeutics, and reactive oxygen species (ROS). DSBs can also be indirectly generated by reactive oxygen species (ROS). All such DSB

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1064-3745 ips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Base Excision Repair Pathway: Methods and Protocols. serves as a valuable r978-1-0716-3375-5978-1-0716-3373-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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