Titlebook: Bacterial Regulatory RNA; Methods and Protocol Véronique Arluison,Claudio Valverde Book 2024Latest edition The Editor(s) (if applicable) an

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In Vitro Methods for the Investigation of sRNA-mRNA Interactions in he localization of minimal regulatory regions of an sRNA, the determination of the secondary structures of both interacting RNAs and their complex as well as the analysis of RNA chaperones that may promote the sRNA/mRNA interaction.

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Extraction and Purification of Outer Membrane Vesicles and Their Associated RNAsia with the environment. OMVs act as information carriers and encapsulate various molecules such as proteins, lipids, metabolites, and RNAs. OMVs and sRNAs play a broad range of functions from pathogenesis to stress resistance, to biofilm formation and both mediate interkingdom signaling. Various st

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Analysis of Phage Regulatory RNAs: Sequencing Library Construction from the Fraction of Small Prokaried. The first microRNA-size functional ribonucleic acid occurring in a bacterial cell has been described only recently, and it was found to be encoded by a bacteriophage. One of the reasons for such a scarcity in this field is the lack of procedures intended for the isolation and selection of molec

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Ribosome Profiling Methods Adapted to the Study of RNA-Dependent Translation Regulation in rious stresses encountered in their host environments. . is a well-studied model for RNA-mediated regulation of virulence and pathogenicity, with sRNAs playing significant roles in shaping . interactions with human and animal hosts. By modulating the translation and/or stability of target mRNAs, sRN

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CRISPR Interference-Based Functional Small RNA Genomicsns a major bottleneck. Gene inactivation through random transposon insertion has proven extremely valuable in discovering hidden gene functions. However, this approach is biased toward long genes and usually results in the underrepresentation of sRNA mutants. In contrast, CRISPR interference (CRISPR