Titlebook: Bacterial Polysaccharides; Methods and Protocol Inka Brockhausen Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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Conjugation of Synthetic Oligosaccharides to Proteins by Squaric Acid Chemistry,quarate derivative of such oligosaccharide is formed first, which is followed by its reaction with a protein carrier. Monitoring of the conjugation reaction is achieved by SELDI-TOF-MS or MALDI-TOF-MS. This experimentally simple procedure yields desired glycoconjugates in high yields and with reproducible hapten–protein ratios.

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