Titlebook: Bacterial Persistence; Methods and Protocol Natalie Verstraeten,Jan Michiels Book 2021Latest edition The Editor(s) (if applicable) and The

大約冬季

Maria Montessoris p?dagogischer Reformimpulsstence. We describe a method of single-cell imaging using glass bottom dishes and a nutrient agarose pad that allows for long-term single-cell microscopy observation in a stable environment. We apply this method to characterize the lag phase and persistence of individual . cells.

天賦

莊嚴

Helmut Niegemann,Armin Weinbergerin persister cells, which are isolated by treatment with beta-lactam antibiotics. Furthermore, persister proteomes during the postantibiotic recovery phase are analyzed by label-free quantification. The presented methods are valuable tools to shed light on persister physiology.

Emasculate

Biographie – Genealogie – Generationterest in the scientific community. However, a framework on how this research should be conducted is currently lacking. Therefore, we here present a method to detect and validate genes important for persister awakening.

獨白

Observing Bacterial Persistence at Single-Cell Resolutionstence. We describe a method of single-cell imaging using glass bottom dishes and a nutrient agarose pad that allows for long-term single-cell microscopy observation in a stable environment. We apply this method to characterize the lag phase and persistence of individual . cells.

上流社會

高腳酒杯

Analyzing Persister Proteomes with SILAC and Label-Free Methodsin persister cells, which are isolated by treatment with beta-lactam antibiotics. Furthermore, persister proteomes during the postantibiotic recovery phase are analyzed by label-free quantification. The presented methods are valuable tools to shed light on persister physiology.

兇殘

ornithology

https://doi.org/10.1007/978-3-531-92015-3subsequent microscopic analyses. We here describe a protocol to enrich persisters in a culture using β-lactam-induced filamentation followed by size separation. This protocol minimizes the amount of cell debris in the final sample, facilitating single-cell studies of persister cells.

果核