Titlebook: Bacterial Persistence; Methods and Protocol Jan Michiels,Maarten Fauvart Book 2016 Springer Science+Business Media New York 2016 Antibiotic

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Springer Reference Sozialwissenschaftenal ancestral level up to 100?%. This method will help to unravel molecular pathways to persistence, and opens up a myriad of new possibilities in persister research, such as the convenient study of nearly pure persister cultures and the possibility to investigate the role of time and environmental aspects in the evolution of persistence.

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Optimized Method for Measuring Persistence in Escherichia coli with Improved Reproducibilityata around the mean, making interpretation difficult. Furthermore, additional reproducibility problems arise from the lack of a standard method, different research groups using different protocols. Here, we describe a standard and optimized method for monitoring . persister cells at the population level allowing for maximal reproducibility.

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Protocol for Determination of the Persister Subpopulation in , Biofilms. Upon incubation, the fraction of surviving biofilm cells is determined by plating and plotted versus the used concentrations of the antifungal compound. If a persister subpopulation in the biofilm is present, the dose-dependent killing of the biofilm cells results in a biphasic killing pattern.

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1064-3745 ation advice from the experts.This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in .Escherichia coli .cultures, pro

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Iris Pfeiffer,Simone Kaiser,Manon Rani Sharmompounds on biofilms. Stainings to evaluate the amount of biomass (crystal violet staining) and the number of metabolically active cells (resazurin assay) are discussed and specific attention is paid to the use of this model to quantify persisters in sessile populations.

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