Titlebook: Bacterial Pangenomics; Methods and Protocol Alessio Mengoni,Marco Galardini,Marco Fondi Book 2015 Springer Science+Business Media New York

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Methods for Assembling Reads and Producing Contigs,wadays it is an almost routine practice in many molecular biology labs. In this chapter we discuss in depth the various methods to assemble the short sequences (called reads) obtained from a massive sequencing system, using different software and strategies, and how to perform some fundamental quality controls on the data obtained.

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Book 2015ucible protocols and notes on troubleshooting and avoiding known pitfalls..Authoritative and easily accessible, .Bacterial Pangenomics: Methods and Protocols. will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experi

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https://doi.org/10.1007/978-3-658-25978-5Further tests examining growth in various media are used to distinguish secondary chromids from plasmids, and mutational analysis (e.g., using the yeast FLP/FRT recombination system) is employed to identify essential genes carried by particular chromids.