Titlebook: Bacterial Multidrug Exporters; Methods and Protocol Akihito Yamaguchi,Kunihiko Nishino Book 2018 Springer Science+Business Media, LLC 2018

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Molecular Modeling of Multidrug Properties of Resistance Nodulation Division (RND) Transportersn Gram-negative bacteria. Structural information on AcrAB-TolC and MexAB-OprM, major efflux pumps of . and . respectively, boosted intensive research aimed at understanding the molecular mechanisms ruling the active extrusion processes. In particular, several studies were devoted to the understandin

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https://doi.org/10.1007/978-1-4939-7454-2Multidrug-resistant pathogens; Bacterial tolerance against antibiotics; Drug resistance; Crystal struct

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Akihito Yamaguchi,Kunihiko NishinoIncludes cutting-edge methods and protocols for the study of bacterial multidrug exporters.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180302.jpg

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Alternsforschung: Entwicklung und Tendenzen sample quality is the most important parameter that should be addressed. In almost every case, highly pure, monodisperse, and stable protein sample is a prerequisite. Vapor diffusion is in general the method of choice for obtaining crystals. Here, we discuss a detailed protocol for overproduction a

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Allmacht, Allwissenheit und Allgütem in cellular level. These pumps not only confer multidrug resistance of cancer cells and pathogenic bacteria but also cause hereditary diseases through the mutation. Our purposes are to elucidate the molecular structures and mechanisms of these xenobiotic exporters..We had succeeded to determine th

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https://doi.org/10.1007/978-3-476-04735-9on of this technique is not straightforward and often hampered by the difficulties associated with expression, purification, and crystallization. Here we present our protocol and methodology for crystallizing the CusBA adaptor–transporter complex of .. Using these procedures, we were able to produce

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