Titlebook: Bacterial Chromatin; Methods and Protocol Remus T. Dame Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 nucleo

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Determination of the 3D Genome Organization of Bacteria Using Hi-Cal genomes are organized into insulated domains similar to the Topologically Associating Domains (TADs) detected in eukaryotic cells. Chromosome conformation capture (3C) technologies are used to analyze in vivo DNA proximity based on ligation of distal DNA segments crossed-linked by bridging protei

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Genomic SELEX Screening of Regulatory Targets of , Transcription Factorsf protein-protein interaction: at the first step, seven species of the sigma subunit are involved, at the second step, a total of approximately 300 species of transcription factor (TFs). For the identification of the regulatory targets of these two groups of regulatory proteins, we developed two in

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Modular Assembly of Synthetic Secondary Chromosomesrch and biotechnological applications. Questions of how chromosomes need to be constructed to maintain the genetic information can now be answered by a learning-by-building approach. Here, we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in . based on