Titlebook: BCL-2 Family Proteins; Methods and Protocol Evripidis Gavathiotis Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

癡呆

Cryo-Electron Microscopy to Study Bax Pores and MOMP,ystems—outer membrane vesicles (OMVs) and liposomes—to visualize the pores in the membrane by using cryo-electron microscopy (cryo-EM). We have revealed the morphology of the pore, determined the localization of Bax labeled with nanogold and have performed image analysis to help understand the mechanisms of pore formation induced by Bax.

跟隨

Rapid Imaging of BCL-2 Family Interactions in Live Cells Using FLIM-FRET,al data acquisition. We developed an automated optical system with eight parallel detectors for rapid and efficient data collection. Here we describe how to use this system for FLIM-FRET imaging of Bcl-2 family protein interactions in a 384-well plate format.

AV-node

表狀態(tài)

MAG

Headstrong

amplitude

Overview of BCL-2 Family Proteins and Therapeutic Potentials,ein family, both literally and figuratively. BH3 motifs are present in antiapoptotic and proapoptotic BCL-2 homologs, and in a separate group of unrelated BH3-only proteins often appended to the BCL-2 family. BH3-containing helices mediate many of their physical interactions to determine cell death

peritonitis

匍匐前進(jìn)

Application of Mito-Priming to Generate BCL-2 Addicted Cells,gement of mitochondrial outer membrane permeabilization or MOMP to initiate cell death. We have developed a new method, called mito-priming, that allows for rapid and synchronous induction of mitochondrial apoptosis in an on-target manner. Mito-priming uses coexpression of pro- and antiapoptotic Bcl

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