Titlebook: B Cell Receptor Signaling; Methods and Protocol Chaohong Liu Book 2018 Springer Science+Business Media, LLC 2018 Drosophila.S2 Schneider ce

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Single-Particle Tracking of Cell Surface Proteins,cell surface proteins. These studies provide fundamental insights into the regulation of membrane receptor activation and the assembly of signaling clusters. Here, we describe a method to label and track B cell receptor (BCR) and other cell surface proteins and how this method can be adapted to simu

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,Time-Lapse F?rster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for AnalET imaging by the sensitized acceptor emission method has been widely applied to study the dynamical association between two molecules at a nanometer scale in live cells. Here, we provide a detailed protocol for FRET imaging by sensitized emission using a confocal laser scanning microscope to analyz

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Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation,ymerization of actin monomers into filaments provides the force required for B cell spreading on the antigen-presenting cell (APC). Interestingly, the actin network also participates in cellular signaling at multiple levels. Fluorescence microscopy plays a critical role in furthering our understandi