Titlebook: B Cell Receptor Signaling; Methods and Protocol Chaohong Liu Book 2018 Springer Science+Business Media, LLC 2018 Drosophila.S2 Schneider ce

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978-1-4939-8497-8Springer Science+Business Media, LLC 2018

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Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution,n induce other mutations, and plays a central role in introducing diversification of the antibody repertoire in B cells. Ectopic expression of AID in bacteria and non-B cells can also lead to frequent mutations in highly expressed genes. Taking advantage of this feature of AID, in recent years, syst

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Analyzing Mouse B Cell Responses Specific to LCMV Infection,anding the basic principles of the B cell responses during viral infection is of substantial importance for anti-viral vaccine development. In inbred mice, lymphocytic choriomeningitis virus (LCMV) infection elicits robust and typical T cell-dependent B cell responses, including germinal center reac

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Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors,memory cells and plasma cells. Expressing genes in altered versions in primary B cells and B cell lines is an important approach to understanding how B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to de

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DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigthe B cell receptor (BCR), which recognizes and binds antigen displayed on the surface of an antigen-presenting cell (APC). To acquire the antigen, B cells use myosin contractility to physically pull BCR-antigen clusters from the APC membrane. These mechanical forces influence association and dissoc

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