Titlebook: B Cell Protocols; Hua Gu,Klaus Rajewsky Book 2004 Humana Press 2004

Keratectomy

Davide Maltoni,Dario Maio,Salil Prabhakarf BrdU for short-term labeling experiments. Using the protocol described, it is possible to combine flow cytometric detection of incorporated BrdU simultaneously with fluorescence staining of various cell surface markers.

observatory

1064-3745 iology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side w

議程

Fingerprint Analysis and Representation,e targeting, to in vitro manipulate embryonic stem (ES) cells and to rapidly identify successfully targeted ES colonies. It also provides an updated list of Cre strains currently used to assess gene function at defined stages of B-cell development and guidelines to generate single-copy, knock-in transgenes regulated in a Cre-dependent manner.

giggle

Fingerprint Analysis and Representation,coding either normal or blocking forms of experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it possible to study the effects of these proteins on late stages of B-cell development, including proliferation, class switch recombination, and plasmacytic differentiation.

相容

https://doi.org/10.1007/978-3-319-76526-6evelopment. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreover, the protocols of how to grow and to test the plasticity of .-deficient pre-B-1 cells are also outlined.

統(tǒng)治人類

ureter

Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors,coding either normal or blocking forms of experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it possible to study the effects of these proteins on late stages of B-cell development, including proliferation, class switch recombination, and plasmacytic differentiation.

輪流

receptors

BAIL

Characterization of B-Cell Maturation in the Peripheral Immune System,ry, analyzing the relative expression of several developmentally regulated markers. We describe the method for the staining of the cells and the analysis of the collected data and show examples of the results obtained in normal and mutant mice.