Titlebook: Axon Regeneration; Methods and Protocol Ava J. Udvadia,James B. Antczak Book 2023 The Editor(s) (if applicable) and The Author(s), under ex

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Analysis of Axonal Regrowth and Dendritic Remodeling After Optic Nerve Crush in Adult Zebrafish,he brain, using retro- and anterograde tracing experiments and an immunofluorescent staining for presynaptic compartments, respectively. Finally, methods to analyze RGC dendrite retraction and subsequent regrowth in the retina are delineated, using morphological measurements and immunofluorescent staining for dendritic and synaptic markers.

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Translating Ribosome Affinity Purification (TRAP) and Bioinformatic RNA-Seq Analysis in Post-metamoof the experimental design and necessary controls and their rationale, along with a description of the bioinformatic steps involved in analyzing the . translatome using TRAP and RNA-Seq, is also provided.