Titlebook: Adult Stem Cells; Methods and Protocol Paolo Di Nardo,Sanjiv Dhingra,Vincenzo Desiderio Book 2024Latest edition The Editor(s) (if applicabl

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Cancer Stem Cells: Detection and Characterization from Solid Tumors, CSCs in tumor formation, which can only be possible by devising perfected methodologies to isolate, detect, and characterize them. In this chapter, we outline the key protocols to culture, identify, and isolate CSCs from solid tumors to further advance basic and clinical investigation related to CSCs and their role in tumor biology.

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Pde5-Inhibitors

Safe and Noninvasive Method for Generating Induced Mesenchymal Stem Cells from Urinary Epithelial Cnical trials for various diseases and injuries, showcasing their therapeutic promise. However, traditional sources of MSCs have limitations in terms of scalability and storage. To address these challenges, this study aims to provide a method of creating an alternative source of induced pluripotent s

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Adult Mesenchymal Stem Cells in Presence of Glycosaminoglycans,articular, scaffolds and/or hydrogel based on glycosaminoglycans (GAGs) play a pivotal role due to their ability to support the in vitro growth and differentiation of MSCs toward a specific phenotype. Here, we describe different possible approaches to develop GAGs-based biomaterials, hydrogel, and p

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A Three-Step Protocol to Differentiate iPSC into Colon Organoids, in vitro tool. They offer highly efficient models for studying both embryonic development and disease progression processes. Colon organoids can also be generated from biopsies obtained during a colonoscopy. However, the invasive nature of biopsy collection poses practical challenges and introduces

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Enriching Cardiomyocytes Derived from hiPSCs by Magnetic-Activated Cell Sorting (MACS),ng disease modeling, regenerative medicine, screening of drug toxicity and investigation of cardiomyogenesis. Despite remarkable improvement in methodology enabling differentiation of hiPSCs into CMs, applied protocols generate heterogeneous cell populations composed of CMs along with differentiated