Titlebook: Atlas of Human Pluripotent Stem Cells in Culture; Lyn Healy,Ludmila Ruban Book 2015 Springer Science+Business Media New York 2015 embryoni

無關(guān)緊要

LUT

Satyaprakash,Ayon K. Tarafdar,Ravi Guptaf, as is the case for retroviral vectors. To date, the methods of choice are those that use agents that do not integrate into the host genome. These include Sendai viral vectors, episomal vectors, protein transduction and RNA-based transfection.

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媽媽不開心

門窗的側(cè)柱

Christopher Chase-Dunn,Andrew Jorgensonnal plating density. Human embryonal carcinoma cells (hECCs), in contrast, can be grown as a monolayer, but when overconfluent, they spontaneously differentiate (Figs. 12.5, 12.6 and 12.7). Both mESC lines and hECC lines are often used as control cell lines for the assessment of stem cell-specific antibodies and gene expression.

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倫理學(xué)

Inactivated Mouse and Human Fibroblasts,m is the system of choice. This chapter looks at the morphology of mouse and human feeder cells in order make a subjective assessment of the quality of the feeders and their suitability to support the growth and expansion of PSCs (Figs. 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8 and 3.9).

LASH

Human Embryonic Stem Cells,yonal carcinoma cells. These cells were shown to be pluripotent in nature through their ability to form teratomas in immunocompromised mice, composed of tissues derived from all three germ layers. The potential utilisation of these cells for disease modelling, toxicology studies and regenerative medicine heralded a new era in the field of biology.

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Derivation of Induced Pluripotent Stem Cells,f, as is the case for retroviral vectors. To date, the methods of choice are those that use agents that do not integrate into the host genome. These include Sendai viral vectors, episomal vectors, protein transduction and RNA-based transfection.