Titlebook: Asthma; Methods and Protocol Magdalena M. Gorska Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Pre - Planetary Disks and Planet Formationcing Lin. population that lacks surface expression of canonical CRTH2 but expresses CD30 and TNFR2. Herein, we describe a detailed protocol for isolation, staining, and analysis of the conventional Lin.CRTH2.IL7Ra. and the non-conventional Lin.CD30.TNFR2. ILC2 populations.

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A Novel Nonhuman Primate Model of Nonatopic Asthma,n primate models of asthma can be spontaneous, induced, or experimentally manipulated by various exposures. Here we describe a model of exacerbation of airway hyperreactivity induced by exposure to an air pollutant, ozone, in a cohort of young adult asthmatic rhesus macaques.

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Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies,ple who smoke, vape, or both. Here, we will describe methods to collect, expand, and culture human airway epithelial cells from endobronchial brushings and expose these cells cultured at the air–liquid interface to cigarette smoke or electronic cigarette vapor.

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Towards a Theory of Business Web Growth,ods that are commonly used in mechanistic studies of asthma in mice. These methods include tracheotomy and bronchoalveolar lavage, cytospin and morphologic analysis of bronchoalveolar lavage cells, and lung harvest and digestion for generation of single-cell suspension.

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Formation and Early Growth of Business Websoncentration. In order to standardize the concentrated RV stock, a plaque assay on HeLa cells is outlined as a method of calibrating infectivity. Once the number of plaque-forming units is determined, the standardized virus is used for mouse infection.

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David N. Schramm,Katherine Freese stimulation of PCLSs with immune mediators such as pro-inflammatory tumor necrosis factor α (TNF-α). These models are useful to investigate mechanisms of virally induced asthma exacerbations and study modes of action and efficacy of antiviral and/or anti-inflammatory drugs.